Langxing Company Limited  

Product Name: Purification Reagents Nucleic Acid Extraction or Purification Kit Transport Package: Carton Specification: 80Tests/kit Origin: China HS Code: 3822009000 Product Description [Product name]Nucleic acid Extraction and Purification Kit[Intended Use]This kit use magnetic bead with a unique separation effect and buffer system to isolate and purify viral nucleic acids from samples such as blood, serum, cells, saliva, swab preservation solution, and tissue homogenate.Transportation & Storage Temperature:R.T temperature(15-25 ºC)Specification:80Tests/kit ;8 kits/case(640 tests/case)[Product Features]1. Samples are widely used: vlrus nucleic acids can be directly extracted from blood, serum, cells, saliva, swab preservationsolution, tissue homogenate and other samples.2. High quality: with a unique lysis buffer system, the purified viral nucleic acid can meet the experimental requirements such ashigh-throughput sequencing.3. Fast and non-toxic: Adopt magnetic bead adsorption, no need to use phenol chloroform, it can be used in combination withautomated instruments, the experiment can be completed within 30-35 min.[Matters needing attention] Please read this note before using.1. Avoid repeated freezing and thawing of samples, otherwise the yield of nucleic acid extraction will decrease.2. Magnetic beads need to be fully mixed on the vortex oscillator before use.3. Perform all steps at room temperature (24°C), unless otherwise noted.4. Do not mix reagents different batches without special instructions, and ensure that the kit is used during the validity period.[Operating steps]1.Sample preparation:a.Blood, serum, saliva and other liquid samples can be directly extracted for viral nucleic acids.b. Tissue samples: Weigh 50-200mg of tissue into a 2mL enzyme-free centrifuge tube, add 0.3-0.8 ml of physiological saline or PBSbuffer solution and 2-4 steel balls, and perform low-temperature grinding in a grinder for 0.5-2 min until no lumps are present, thencentrifuge at 8000 rpm for 1 min, and take the liquid supernatant for nucleic acid extraction.c.Oral swab brush: The oral cells can be lysed and collected in 200-500 μL cell preservation solution or PBS solution for nucleic acidextraction.2. Deep-well plate preparation:Take out the pre-sealed reagent deep-well plate from the kit, mix upside down several times to make the magnetic beads completeresuspension, light shake deep-well plate, so that the reagent and magnetic beads are concentrated to the bottom of deep-well plate (alsouse deep-well plate centrifuge ,500 rpm ×1 min for centrifugation), carefully tear off aluminum foil sealing film before use, avoid causingdeep-well plate vibration and liquid spatter.3. Add the sample: add 200μL of samples to each well in the well position 1.4. Machine operation:a. Put the deep-well plates of station 1, 2, 3, 4 and 6 into the instrument ZK-96 (station 1 ~ station 6 from left to right).b. Insert the magnetic sleeve.c. Select the program K.BD02S on the instrument.d. Start the run, then load the prepared plates into position when prompted by the instrument. 5.The collection of nucleic acid: After protocol is complete, immediately remove the elution solution plate from the instrument and cover the plate or transfer the elution solution or plate of choice for final storage.(The purified nucleic acid is ready for immediate use. Alternatively, store the eluate at -20 °C for long-term storage.)